SeqWell

plexWell™ Library Prep Technology.

Scale & Performance through Iterative Barcoding

Discover the Features and Benefits of “True Multiplexing”

plexWell’s integrated normalizing library preparation technology allows the creation of balanced library pools without the need for sample or library normalization. The simplified 3-hour workflow multiplexes 100 to 1000 samples for loading in a single sequencing run, resulting in better overall sequencing performance.

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Simple and fast

Multiplex 1000’s of samples with ease.

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Normalization

Built-in normalization with every kit.

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Performance

Uniform insert sizes & sample read counts.

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Quality

Improved overall sequencing performance.

Simple Three Step Protocol

Sample Barcoding

Unfragmented DNA is tagged with sample-specific i7-barcoded adapters.

P7 primers and unique i7 indexes are inserted randomly into each DNA sample by a transposon. The same amount of P7 is added to each sample regardless of DNA input amount. This limiting reagent normalizes the samples, which are now pooled into a single tube.

Pool Barcoding

Pooled sample-barcoded DNA is tagged with pool-specific i5-barcoded adapters.

The pool-specific i5 barcoded adapters containing P5-adapters are now added in the second transposition reaction. Excess pool barcoding reagent inserts the same average distance from every sample barcode.

Amp + Purification

Barcoded library fragments are amplified.

The final step is a library amplification with universal primers and final SPRI purification. The pooled library is now ready to be loaded in an Illumina® sequencer to produce normalized or balanced distribution of sequencing reads.

plexWell workflow in action

Explained Technology

The technical foundation of the plexWell approach is an initial reagent-limited tagging step performed on many samples in parallel, coupled with a subsequent pooled library generation step. When applied together, these two steps produce an approximately equal number of sequenceable library fragments from each of a potentially large collection of samples. The two-step process also allows for a large combinatorial repertoire of barcode combinations.

Multiplexing Uniformity

plexWell™ Achieves a Significantly Better Level of Multiplexing Uniformity for Highly Multiplex Sequencing Applications

Featured Workflow

SeqWell

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Reagents

I-DOT

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Liquid handling

C.WASH™

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DNA purification

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Sequencing & Data analysis

Explore your options

Not available in the US, Canada, Australia and New Zealand.

plexWell™

Simple and scalable multiplexed workflow for 1000’s of samples.

scRapid

Premier single cell RNA Sequencing.