C.SIGHT

Single Cell Dispenser

A gentle single cell dispenser with imaged based sorting that isolates individual cells based on morphology and provides visual proof of clonality from day zero. Its compact footprint, simple workflow, and automation-ready design make it an ideal entry point for cell line development laboratories.

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BENEFITS

The Entry Point Into Image-Based Single-Cell Isolation

Dispense-Ready in Minutes

With minimal setup and intuitive software, the system is ready to begin single-cell isolation in just a few minutes.

Morphology-based cell selection

Automatically classify and dispense cells based on size, shape, and roundness.

Fast and Efficient

Single cells are gently dispensed into a 96-well plate in under 2 minutes or into a 384-well plate in under 8 minutes.

Gentle Piezo Dispensing

Low-shear piezo dispensing helps preserve cell viability while accurately isolating individual cells for downstream culture.

Compact Design

Designed to fit inside standard biosafety cabinets, allowing sterile single-cell isolation without sacrificing valuable bench space.

Image-based dispensing

Captures nozzle images during every dispensing to verify single-cell dispensing events. For double-assured monoclonality assurance, consider the UP.SIGHT.

More Clones. Fewer Plates. Less Time.

With >97% single-cell dispensing efficiency, up to 80% CHO cell outgrowth, and dispensing speeds of up to 40 cells per minute, C.SIGHT helps laboratories generate more viable clones while reducing hands-on time and consumable usage.

This comparison illustrates the number of plates and time required to generate 500 clones using different single-cell isolation methods.

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CLD _ Cell Sorting and Single-Cell Efficiency Infographic

From One Million Cells, to the One Clone in a Million

High performance in all relevant single-cell dispensing parameters means that isolating low frequency, high value clones becomes deterministic. Predictable success supports delivering CLD projects on time and on budget.

FEATURES

Why Researchers Choose C.SIGHT

Fast Setup & Throughput

Pressing UPSIGHT Button

A Full Campaign in Mere Hours

C.SIGHT is dispense-ready in under five minutes with virtually no setup before starting a dispensing run at around 40 single cells a minute. A simple sample dilution to 10E6 cells/mL suffices.

A 96-well plate is seeded in 2 minutes, or a 384-well plate in 8 minutes, with single-cell dispensing efficiencies exceeding 97%. Cleaning and shutdown take only an additional 5 minutes.

 

No Risk of Cross-Contamination

EASYON-Cartridge-compress (1)

Maintain Sample Integrity

C.SIGHT is a compact system that fits neatly inside a standard biosafety cabinet. Cell suspensions are loaded into individually packaged EASY.ON single-use cartridges that prevent cross-contamination between samples.

 

Built for Confidence

CYT _ Confidence 3 (1)

Regulatory & Industry Adoption

C.SIGHT uses the same image-based dispensing technology used in the UP.SIGHT and that is the gold standard for monoclonality documentation and IND filings.

The system is 21 CFR Part 11 and GMP compatible, and widely adopted in mAb manufacturing and hiPSC-based allogeneic cell therapy facilities.

 

Proof from Day 0

CYT | Day0

Visual Proof of Clonal Origin

Unlike impedance, FACS, or limiting dilution, which infer clonality statistically, C.SIGHT provides direct visual confirmation of clonal origin at Day 0.

With the odds of an unnoticed doublet at roughly 1 in 2 million dispensing events, researchers gain confidence from the very first step of clone development.

 

 

 

Pressing UPSIGHT Button

A Full Campaign in Mere Hours

C.SIGHT is dispense-ready in under five minutes with virtually no setup before starting a dispensing run at around 40 single cells a minute. A simple sample dilution to 10E6 cells/mL suffices.

A 96-well plate is seeded in 2 minutes, or a 384-well plate in 8 minutes, with single-cell dispensing efficiencies exceeding 97%. Cleaning and shutdown take only an additional 5 minutes.

 

EASYON-Cartridge-compress (1)

Maintain Sample Integrity

C.SIGHT is a compact system that fits neatly inside a standard biosafety cabinet. Cell suspensions are loaded into individually packaged EASY.ON single-use cartridges that prevent cross-contamination between samples.

 

CYT _ Confidence 3 (1)

Regulatory & Industry Adoption

C.SIGHT uses the same image-based dispensing technology used in the UP.SIGHT and that is the gold standard for monoclonality documentation and IND filings.

The system is 21 CFR Part 11 and GMP compatible, and widely adopted in mAb manufacturing and hiPSC-based allogeneic cell therapy facilities.

 

CYT | Day0

Visual Proof of Clonal Origin

Unlike impedance, FACS, or limiting dilution, which infer clonality statistically, C.SIGHT provides direct visual confirmation of clonal origin at Day 0.

With the odds of an unnoticed doublet at roughly 1 in 2 million dispensing events, researchers gain confidence from the very first step of clone development.

 

 

 

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KEY RESOURCES

Validated Across Diverse Single-Cell Workflows

C.SIGHT is the versatile solution for gentle, label-free single-cell isolation across cell line development, cell therapy, stem cell research, and single-cell omics. The following resources demonstrate how researchers apply microfluidic image-guided dispensing to achieve high cell viability and documented sorting.

BLOG

Single-Cell Seeding: From Basics to Advanced Methods

We discuss the different methods of single-cell seeding and monitoring, highlighting the relevant advantages and disadvantages and looking to the future of the single-cell seeding process.

 

Read Now →

BLOG

Cell Line Development: Comprehensive Insights and Advances

This article covers applications, innovations, and challenges in cell line development and introduces CYTENA’s instruments as future-proof solutions for therapy development.

 

Read Now →

Related Applications

Cell-Line-Development

CELL LINE DEVELOPMENT

Selection and cultivation of stable clonal lines for biomanufacturing.

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CELL THERAPY & STEM CELLS

Cellular therapeutics, stem cell biology, and advancements in regenerative medicine.

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SINGLE-CELL OMICS

High-resolution genomic and transcriptomic analysis of cellular heterogeneity.

Related Products

F.SIGHT

Dual-channel fluoroscent and brightfield sorting for precise isolation of labeled cells.

UP.SIGHT

Single-cell dispenser with fluorescence and plate imaging for double-assured monoclonality.

C.STATION

Automate cell line development–integrating single-cell isolation, imaging and clone selection into one efficient, compliant workflow.

B.SIGHT

High-precision single-cell dispensing for bacteria, yeast, and other microbes.

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