Publications
Peer-reviewed research that pushes the
boundaries of the life sciences community.

Publications
Peer-reviewed research that pushes the
boundaries of the life sciences community.

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Romy Kronstein-Wiedemann, Jessica Thiel, Duran Sürün, Madeleine Teichert, Stephan R. Künzel, Stefan Zimmermann, Lisa Wagenführ, Frank Buchholz, Torsten Tonn
Jun, 21 2024

SPEED-MODE cell line development (CLD): Reducing Chinese hamster ovary (CHO) CLD timelines via earlier suspension adaptation and maximizing time spent in the exponential growth phase
Biotechnology Progress
Kavya Ganapathy, Cynthia Lam, Joni Tsukuda, Alyssa Sargon, Adrian Nava, Peter Harms, Amy Shen, Gavin Barnard, Shahram Misaghi
May, 8 2024

When will we have a clone? An industry perspective on the typical CLD timeline
Biotechnology Progress
Howard Clarke, Anke Mayer-Bartschmid, Chenxing Zheng, Elizabeth Masterjohn, Falguni Patel, Mark Moffat, Qingxiang Wei, Ren Liu, Robyn Emmins, Simon Fischer, Stephanie Rieder, Thomas Kelly
Mar, 13 2024

Label-free isolation and deposition of single bacterial cells from heterogeneous samples for clonal culturing
Scientific Reports
J. Riba, T. Gleichmann, S. Zimmermann, R. Zengerle & P. Koltay
Dec, 5 2023

The stress sensor GCN2 differentially controls ribosome biogenesis in colon cancer according to the nutritional context
Molecular Oncology
Marie Piecyk, Mouna Triki, Pierre-Alexandre Laval, Cedric Duret, Joelle Fauvre, Laura Cussonneau, Christelle Machon, Jerôme Guitton, Nicolas Rama, Benjamin Gibert, Gabriel Ichim, Frederic Catez, Fleur Bourdelais, Sebastien Durand, Jean-Jacques Diaz, Isabelle Coste, Toufic Renno, Serge N. Manié, Nicolas Aznar, Stephane Ansieau, Carole Ferraro-Peyret, Cedric Chaveroux
Jul, 15 2023

A new T-antigen negative HEK293 cell line with improved AAV productivity
Biotechnology and Bioengineering
Coralie Croissant, Joshua Armitano, Bertrand Lazuech, Danijel Švec, Cyril Pugin, Anaïs Guesdon, Louise Bryan, Antonio Castro, Léa Neuhaus, Giulia Fonti, Jacopo Martinis, Maria J. Wurm, Florian M. Wurm, Paco Pino
May, 26 2023

Unveiling the main factors triggering the coagulation at the SiC-blood interface
Journal of Biomedical Materials Research
Zümray Vuslat Parlak, Norina Labude-Weber, Kerstin Neuhaus, Christina Schmidt, Aaron David Morgan, Rafał Zybała, Jesus Gonzalez-Julian, Sabine Neuss, Karolina Schickle
Mar, 16 2023

A Monoclonal Human Alveolar Epithelial Cell Line (“Arlo”) with Pronounced Barrier Function for Studying Drug Permeability and Viral Infections
Advanced Science
Patrick Carius, Annemarie Jungmann, Marco Bechtel, Alexander Grißmer, Annette Boese, Gilles Gasparoni, Abdulrahman Salhab, Ralf Seipelt, Klaus Urbschat, Clémentine Richter, Carola Meier, Denisa Bojkova, Jindrich Cinatl, Jörn Walter, Nicole Schneider-Daum, Claus-Michael Lehr
Feb, 7 2023

Blastic transformation of BCR-ABL1 positive chronic myeloid leukaemia through acquisition of CBFB-MYH11 and mutant KIT
British Journal of Haematology
Shoumariyeh K, Hussung S, Niemöller C, Bleul S, Veratti P, Follo M, Riba J, Philipp U, Palmer JM, Pfeifer D, Pantic M, Meggendorfer M, Hackanson B, Finke J, Haferlach T, Duyster J, Miething C, Becker H, von Bubnoff N
Nov, 23 2022

Gene mutations and clonal architecture in myelodysplastic syndromes and changes upon progression to acute myeloid leukaemia and under treatment
British Journal of Haematology
Stosch JM, Heumüller A, Niemöller C, Bleul S, Rothenberg-Thurley M, Riba J, Renz N, Szarc Vel Szic K, Pfeifer D, Follo M, Pahl HL, Zimmermann S, Duyster J, Wehrle J, Lübbert M, Metzeler KH, Claus R, Becker H
Nov, 23 2022

Christoph Ziegenhain # 1, Gert-Jan Hendriks # 1, Michael Hagemann-Jensen 1, Rickard Sandberg
Nov, 23 2022

Rogdakis, T;Charou, D;Latorrata, A;Papadimitriou, E;Tsengenes, A;Athanasiou, C;Papadopoulou, M;Chalikiopoulou, C;Katsila, T;Ramos, I;Prousis, KC;Wade, RC;Sidiropoulou, K;Calogeropoulou, T;Gravanis, A;Charalampopoulos, I;
Jun, 3 2022

Coupling CRISPR interference with FACS enrichment: New approach in glycoengineering of CHO cell lines for therapeutic glycoprotein production
Biotechnology journal
Katja Glinšek, Lovro Kramer, Aleksander Krajnc, Eva Kranjc, Nina Pirher, Jaka Marušič, Leon Hellmann, Barbara Podobnik, Borut Štrukelj, David Ausländer, Rok Gaber
Apr, 28 2022

Recent advances in CHO cell line development for recombinant protein production
Drug Discovery Today: Technologies
Tihanyi, B;Nyitray, L;
Apr, 1 2022

Anaerobic single-cell dispensing facilitates the cultivation of human gut bacteria
Environmental microbiology
Afrizal Afrizal, Thomas C. A. Hitch, Alina Viehof, Nicole Treichel, Thomas Riedel, Birte Abt, Eva M. Buhl, Dietrich Kohlheyer, Jörg Overmann, Thomas Clavel
Mar, 1 2022

Targeting KRAS Regulation with PolyPurine Reverse Hoogsteen Oligonucleotides
International journal of molecular sciences
Psaras, AM;Valiuska, S;Noé, V;Ciudad, CJ;Brooks, TA;
Feb, 14 2022

Automated analysis of genomic sequences facilitates high-throughput and comprehensive description of bacteria
ISME Communications
Hitch, T;Riedel, T;Oren, A;Overmann, J;Lawley, T;Clavel, T;
Dec, 1 2021

Next steps after 15 stimulating years of human gut microbiome research
Microbial biotechnology
Thomas Clavel, Hans-Peter Horz, Nicola Segata, Maria Vehreschild
Nov, 24 2021

Technologies for Single-Cell Printing and Patterning
Handbook of Single-Cell Technologies
Ambhorkar, P;Sakr, M;Kumar, H;Kim, K;
Oct, 29 2021

Coupling picodroplet microfluidics with plate imaging for the rapid creation of biomanufacturing suitable cell lines with high probability and improved multi-step assurance of monoclonality
Biotechnology Journal
Leon P. Pybus, Devika Kalsi, Joe T. Matthews, Ellie Hawke, Nicholas Barber, Rachel Richer, Alison Young, Fay L. Saunders
Oct, 11 2021

Quantitation of amiodarone and N-desethylamiodarone in single HepG2 cells by single-cell printing-liquid vortex capture-mass spectrometry
Analytical and bioanalytical chemistry
Cahill, JF;Kertesz, V;
Oct, 1 2021

High-throughput and automation advances for accelerating single-cell cloning, monoclonality and early phase clone screening steps in mammalian cell line development for biologics production
Biotechnology progress
Vijay Tejwani, Minal Chaudhari, Toyaj Rai, Susan T. Sharfstein
Sep, 3 2021

Printing Microbial Dark Matter: Using Single Cell Dispensing and Genomics to Investigate the Patescibacteria/Candidate Phyla Radiation
Frontiers in Microbiology
Wiegand, S;Dam, HT;Riba, J;Vollmers, J;Kaster, AK;
Jun, 16 2021

MiMiC: a bioinformatic approach for generation of synthetic communities from metagenomes
Microbial biotechnology
Neeraj Kumar, Thomas C. A. Hitch, Dirk Haller, Ilias Lagkouvardos, Thomas Clavel
Jun, 3 2021

Early-Life Immune System Maturation in Chickens Using a Synthetic Community of Cultured Gut Bacteria
mSystems
Zenner, C;Hitch, TCA;Riedel, T;Wortmann, E;Tiede, S;Buhl, EM;Abt, B;Neuhaus, K;Velge, P;Overmann, J;Kaspers, B;Clavel, T;
May, 18 2021

A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells
Nature Methods
Yu Chen, Carlos A. Tristan, Lu Chen, Vukasin M. Jovanovic, Claire Malley, Pei-Hsuan Chu, Seungmi Ryu, Tao Deng, Pinar Ormanoglu, Dingyin Tao, Yuhong Fang, Jaroslav Slamecka, Hyenjong Hong, Christopher A. LeClair, Sam Michael, Christopher P. Austin, Anton Simeonov & Ilyas Singeç
May, 3 2021

Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
Communications biology
Niemöller, C;Wehrle, J;Riba, J;Claus, R;Renz, N;Rhein, J;Bleul, S;Stosch, JM;Duyster, J;Plass, C;Lutsik, P;Lipka, DB;Lübbert, M;Becker, H;
Feb, 1 2021

Design and Evaluation of a Tethered, Open Port Sampling Interface for Liquid Extraction-Mass Spectrometry Chemical Analysis
Journal of the American Society for Mass Spectrometry
Walton, CL;Kertesz, V;Cahill, JF;
Jan, 6 2021

Rapid Lentiviral Vector Producer Cell Line Generation Using a Single DNA Construct
Molecular therapy. Methods & clinical development
Chen, YH;Pallant, C;Sampson, CJ;Boiti, A;Johnson, S;Brazauskas, P;Hardwicke, P;Marongiu, M;Marinova, VM;Carmo, M;Sweeney, NP;Richard, A;Shillings, A;Archibald, P;Puschmann, E;Mouzon, B;Grose, D;Mendez-Tavio, M;Chen, MX;Warr, SRC;Senussi, T;Carter, PS;Baker, S;Jung, C;Brugman, MH;Howe, SJ;Vink, CA;
Dec, 11 2020

Trimeric SARS-CoV-2 Spike Proteins Produced from CHO Cells in Bioreactors Are High-Quality Antigens
Processes
Pino, P;Kint, J;Kiseljak, D;Agnolon, V;Corradin, G;Kajava, A;Rovero, P;Dijkman, R;den Hartog, G;McLellan, J;Byrne, P;Wurm, M;Wurm, F;
Nov, 25 2020

Methods for Automated Single Cell Isolation and Sub-Cloning of Human Pluripotent Stem Cells
Current protocols in stem cell biology
Valeria Fernandez Vallone, Narasimha Swamy Telugu, Iris Fischer, Duncan Miller, Sandra Schommer, Sebastian Diecke, Harald Stachelscheid
Sep, 21 2020

Methods for Single-Cell Isolation and Preparation
Advances in experimental medicine and biology
Pensold, D;Zimmer-Bensch, G;
Sep, 19 2020

Fast Pathogen Identification Using Single-Cell Matrix-Assisted Laser Desorption/Ionization-Aerosol Time-of-Flight Mass Spectrometry Data and Deep Learning Methods
Analytical chemistry
Papagiannopoulou, C;Parchen, R;Rubbens, P;Waegeman, W;
Jun, 2 2020

Single-cell dispensing and ‘real-time’ cell classification using convolutional neural networks for higher efficiency in single-cell cloning
Scientific reports
Riba, J;Schoendube, J;Zimmermann, S;Koltay, P;Zengerle, R;
Jan, 27 2020

Automated System for Small-Population Single-Particle Processing Enabled by Exclusive Liquid Repellency
SLAS technology
Li, C;Niles, DJ;Juang, DS;Lang, JM;Beebe, DJ;
Dec, 1 2019

Assuring Clonality on the Beacon Digital Cell Line Development Platform
Biotechnology Journal
Kim Le, Christopher Tan, Huong Le, Jasmine Tat, Ewelina Zasadzinska, Jonathan Diep, Ryan Zastrow, Chun Chen, Jennitte Stevens
Nov, 19 2019

Simultaneous precise editing of multiple genes in human cells
Nucleic acids research
Riesenberg, S;Chintalapati, M;Macak, D;Kanis, P;Maricic, T;Pääbo, S;
Nov, 4 2019

Effective Acoustic Field Generation in Disposable Dispensing Cartridges for Acoustophoretic Particle Focusin
2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)
Siber, C;Lautscham, L;Schoendube, J;Reichert, P;Stumpf, F;Zimmermann, S;Zengerle, R;Koltay, P;
Jun, 1 2019

Rapid, Untargeted Chemical Profiling of Single Cells in Their Native Environment
Analytical chemistry
Cahill, JF;Riba, J;Kertesz, V;
May, 7 2019

Achieving greater efficiency and higher confidence in single-cell cloning by combining cell printing and plate imaging technologies
Biotechnology progress
Mandy Yim, David Shaw
Oct, 3 2018

Highly parallel microbioreactors for cell line development based on well plates with functional lids
d-nb.info
Cheng-Han, T;
Jan, 1 2018

Evaluation of piggyBac-mediated CHO pools to enable material generation to support GLP toxicology studies
Biotechnology progress
Yashas Rajendra, Sowmya Balasubramanian, Neil A. McCracken, Dawn L. Norris, Zhirui Lian, Matthew G. Schmitt, Christopher C. Frye, Gavin C. Barnard
May, 25 2017

Molecular Genetic Characterization of Individual Cancer Cells Isolated via Single-Cell Printing
PloS one
Riba, J;Renz, N;Niemöller, C;Bleul, S;Pfeifer, D;Stosch, JM;Metzeler, KH;Hackanson, B;Lübbert, M;Duyster, J;Koltay, P;Zengerle, R;Claus, R;Zimmermann, S;Becker, H;
Sep, 22 2016

Technologies for Single-Cell Isolation
International journal of molecular sciences
Gross, A;Schoendube, J;Zimmermann, S;Steeb, M;Zengerle, R;Koltay, P;
Jul, 24 2015

Single-cell PCR of genomic DNA enabled by automated single-cell printing for cell isolation
Biosensors & bioelectronics
Stumpf, F;Schoendube, J;Gross, A;Rath, C;Niekrawietz, S;Koltay, P;Roth, G;
Jul, 15 2015

Enhanced single-cell printing by acoustophoretic cell focusing
Biomicrofluidics
Leibacher, I;Schoendube, J;Dual, J;Zengerle, R;Koltay, P;
Mar, 1 2015

Single-cell printing based on impedance detection
Biomicrofluidics
Schoendube, J;Wright, D;Zengerle, R;Koltay, P;
Jan, 1 2015