F.SIGHT™ OMICS
As the Genomics field pushes forward with more in-depth analysis, innovative methods including the miniaturization of library preparation chemistry have been introduced. This new methodology aims to improve the quality of sequencing while reducing overall costs.
Building on the previous generation of single-cell dispensers and as a purpose-built device, the F.SIGHT OMICS continues CYTENA’s customer-oriented product design. This device has precise cell deposition thanks to the new Automated Offset Correction (AOC) system, where single cells are isolated in PCR plates, in sub microliter droplets, thus enabling downstream miniaturization for NGS Library Preparation. Additionally, with the F.SIGHT OMICS fluorescent sorting, only labelled cells of interest can be isolated for subsequent downstream analysis.
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Key Features & Benefits
Dual Imaging System
Overlay both brightfield and fluorescent images for precise identification of your cells of interest.
No risk of contamination
Eliminate the risk of cross contamination with single-use EASY.ON cartridges.
Downstream miniaturization
Dispense cells in picolitre droplets into the center of the well in 384-well PCR plates, enabling downstream miniaturization with lysis buffer volumes less than 1 μL.
Fast single-cell dispenser
Isolate an entire 384-well plate with single-cells in under 8 minutes.
Assurance of single-cell isolation
Guarantee monoclonality and single-cell isolation with nozzle images that are recorded for every dispensing.
Minimize cell loss
Capture rare cells with Cell Focusing technology that aligns cells at the center of the dispensing cartridge for superior cell detection.
Product Details
Product
Applications
Fluorescent single-cell isolation
Single-cell sorting for NGS library preparation
Single-cell proteomics
Single-cell transcriptomics
Single-cell multiomics
Featured Workflow
With the F.SIGHT OMICS, single cells are Isolated in 0.2nL droplets and, thanks to the Automated
Offset Correction system, are precisely dispensed at the very bottom of PCR plates, facilitating
downstream miniaturization in sub-microliter volumes.
Product Datasheet
Samples | Eukaryotic cells |
Cell size | 5 – 40 µm |
Sorting | Cell morphology and fluorescence |
Droplet volume | ~200 pL |
Substrate | 96- and 384-well plates |
Nozzle imaging | Camera CMOS Magnification 10x Dual-channel: Brightfield and fluorescence |
Fluorescence nozzle imaging | Ex. 488 nm Em. 520 +/- 36 nm |
Precise dispensing into the center of PCR wells | Automated Offset Correction (AOC) |
Processing times | Single-cell dispensing 96-well plate: ~ 2 min Single-cell dispensing 384-well plate: ~ 8 min |
Cartridge | EASY.ON 40 µm disposable cartridge to avoid cross-contamination |
Automation | Lid opening and closing Compatible with standard automation arm and gripper Compatible with third-party automation clients |
Embedded computer | Windows |
Compatibility | Standard biosafety cabinets class 2 |
Footprint | 635 x 400 x 286 mm |
Weight | 40 kg |
Certification | CE, CB, UL (TÜV), RoHS |