UP.SIGHT
Single-cell dispenser with fluorescence and plate imaging for double-assured monoclonality.
-
Time to pure culture cut from 17 to 5 days
-
87% colony growth across wells
-
52.2% OTU richness recovered (vs. 35.2% with traditional methods)
-
87% of abundant taxa (>1% relative abundance) captured
-
82% cumulative relative abundance of faecal OTUs recovered
-
6 novel taxa isolated, including high-priority "most wanted" strains
In short: The B.SIGHT™ workflow delivers faster, more efficient, and more comprehensive microbial isolation, enabling researchers to access valuable bacterial and yeast strains that are difficult to obtain with traditional approaches.
Visual Proof of Clonality
Every isolate begins as a single, imaged cell. Its clonal origin is traceable from the first well.
Selection of Fluorescent Cells
Include or exclude labelled cells by green fluorescence (488 nm laser; 561 nm on request), alongside cell size and roundness. B.SIGHT sorts on multiple parameters at once, filtering out debris, doublets, and unlabelled cells before dispensing. Inquire for alternative fluorescence options.
Gentle, with Scalable Throughput
The B.SIGHT isolates ~40 cells per minute at 97% single-cell efficiency. Unlike FACS, its low-shear dispensing preserves cell viability, reaching up to 80% of wells showing clonal growth.
Automation Ready
Once dispensing is complete, the B.SIGHT automatically releases the multiwell plate and opens the lid, allowing robotic arms to transfer it downstream for further processing in an automated workstation.
No Risk of Cross Contamination
The B.SIGHT fits in a biosafety cabinet and uses single-use cartridges to prevent cross-contamination between samples.
Every isolate begins as a single, imaged cell. Its clonal origin is traceable from the first well.
Include or exclude labelled cells by green fluorescence (488 nm laser; 561 nm on request), alongside cell size and roundness. B.SIGHT sorts on multiple parameters at once, filtering out debris, doublets, and unlabelled cells before dispensing. Inquire for alternative fluorescence options.
The B.SIGHT isolates ~40 cells per minute at 97% single-cell efficiency. Unlike FACS, its low-shear dispensing preserves cell viability, reaching up to 80% of wells showing clonal growth.
Once dispensing is complete, the B.SIGHT automatically releases the multiwell plate and opens the lid, allowing robotic arms to transfer it downstream for further processing in an automated workstation.
The B.SIGHT fits in a biosafety cabinet and uses single-use cartridges to prevent cross-contamination between samples.
“The instrument is handy and super user-friendly. The machine is ready to sort after a quick quality control step and adaption of sort settings. It sorts single cells reliably. Due to the usage of cartridges cross-contamination is not an issue anymore and cells are sorted alive and happy since they are kept in their needed buffer during the entire sort process. An in-built laser enables us to differentiate between fluorescently labelled and unlabelled cells, which makes the whole cell sorting process even more precise.”
“EPFL has developed a new pipeline named parSEQ, designed with the help of CYTENA's B.SIGHT, which aims to optimize the capture of sequence-function datasets from screened protein variant pools. With parSEQ, the functional data of each screened variant is effectively paired with its corresponding sequence data.”
“Our lab uses the B.SIGHT to sort microbial cells from environmental samples. It has a very intuitive user interface and the product managers at CYTENA have been very helpful in optimizing the parameters to suit our needs. It dispenses single cells gently with precision and can fill a 384-well plate in under an hour. The sorting speed and accuracy has greatly maximized our capacity to create large libraries of pure cultures.”
