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B.SIGHT™

The B.SIGHT is a microbial isolation platform that uses cutting-edge optical microscopy and CYTENA’s single-cell dispensing technology to enable isolation of the smallest microbial cells. The second generation B.SIGHT revolutionizes microbiology research by enabling rapid and efficient isolation of bacteria from complex microbiome samples or transformed cultures used for strain development.

It consists of a dispenser chip that rapidly produces microdroplets from the sample inside a disposable cartridge, combined with high resolution optical microscopy and real-time image analysis to guarantee the dispensing of single bacteria onto individual wells of a multiwell plate. Microbial product discovery and development projects can now be accelerated using the B.SIGHT. The device can be integrated into automation workflow and provides a cost effective and high-throughput generation of microbial isolates.

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Key Features & Benefits

Find out how our product can enrich your research

Dual-channel high
resolution microscopy

Detects even the smallest cells based on size and shape. The system comes with full capability to isolate unlabeled and fluorescently labeled single microorganisms.

High-speed
isolation of cells

Accelerate your microorganism workflows with high-speed isolation of single bacteria or yeast into multiwell plates. Process a 96-well plate in 3 minutes and a 384- plate in under 8 minutes.

Assurance
of clonality

Nozzle images confirm the clonality of dispensed single prokaryotic cells.

High
cell viability

The B.SIGHT improves cell viability thanks to fast, gentle and easy-to-use label-free isolation of single bacteria directly into liquid culture or agar.

Compatible with
anaerobic conditions

Small benchtop instrument that integrates into fume hoods and anaerobic chambers. Simply connect the monitor, keyboard and mouse or even use a remote control terminal.

Single-cell genomics of uncultivated bacteria

Dispense single cells from your complex microbiota sample in picolitre droplets directly into the center of the well in 96- or 384-well PCR plates, enabling downstream miniaturization with lysis buffer volumes less than 1 μL.

Automation
ready

The B.SIGHT lid opens automatically, making the device compatible with robotic automation workflows. The instrument also has a small footprint and can easily be placed in most biosafety cabinets.

Product Details

Discover what you can do
Automation ready:
The B.SIGHT automatically releases the multiwell plate after dispensing is complete and automatically opens up the lid, thereby allowing robotic arms to transfer the plate downstream for further processing inside an automated workstation.
Ideal for anaerobic conditions
The B.SIGHT can be operated in an anaerobic chamber thanks to its small footprint, low particle emission and low heat emission. These characteristics provide the ideal environment for isolating strict anaerobes. You can also control the device from outside the cabinet by connecting a monitor, keyboard and mouse, or by using a remote control terminal.
Unprecedented dispensing speed
Isolation of bacteria into multi-well plates is completed in minutes. The B.SIGHT takes only 3 minutes to complete the isolation of bacteria into a 96-well plate and 8 minutes into 384-well plate!
Dual-camera system
The B.SIGHT’s extremely high-resolution optics make even the smallest cells visible. With an innovative and ultrafast dual-camera system, the system can efficiently and rapidly separate cultures of both fluorescent and wild-type bacteria into either liquid culture or agar.
Isolate fluorescent microorganisms
With the B.SIGHT, you can easily isolate fluorescent-labelled cells in minutes.
Specialized for OMICS
The B.SIGHT enables single-cell genomics of microbiome samples without the need for cultivation. The B.SIGHT’s Automated Offset Correction (AOC) allows precise and accurate dispensing of single cells encapsulated in picolitre droplets at the center of wells in PCR plates, thus enabling downstream miniaturization of lysis buffers for NGS library preparation.
Avoid cross contamination:
Isolating cells is simple and contamination free with our disposable EASY.ON cartridges. Simply pipette your sample into the reservoir and mount the cartridge to the B.SIGHT. Microscopic droplets are produced through our bacteria-dispensing cartridges to create stable and accurate bacteria encapsulation. A new sample is then loaded into a new cartridge without a possibility of cross contamination.
Intuitive and easy-to-use software
Use the interactive well plate to design your own unique dispensing experiment and see in real time how each bacterium is sorted through the nozzle of the cartridge and dispensed into 96- or 384-well plates. Images of the dispensed single bacterium are stored on the hard drive, providing proof of clonality.
Wide range of target plates
The device can host a wide range of multiwell plates (96-, 384- and single-well plates) with heights up to 27 mm, enabling the use of low-profile deep well plates.
Isolate more species with the B.SIGHT
List of pure cultures isolated with the B.SIGHT
  • Bacillus subtilis
  • Bacillus vulgatus (strict anaerobe)
  • Enterococcus faecalis
  • Escherichia coli (Top10, DH5alpha, BL21, etc.)
  • Staphylococcus aureus
  • Various cyanobacteria strains
  • Acetobacter
  • Pseudomonas
  • Acinetobacter baumannii
  • Septoria cytisi (fungi)
  • Saccharomyces cerevisiae (yeast)
  • Pichia pastoris (yeast)
List of complex samples processed with the B.SIGHT
  • Human faecal samples
  • Mouse caecal samples
  • Human oral microbiome
  • Wastewater
  • Marine samples
Enabling scientists worldwide

Product
Applications

Accelerate your research

Synthetic biology

Single bacteria isolation

Single yeast isolation

Microbiome research

Anaerobic microorganism isolation

Featured Workflow

With the B.SIGHT, singla cells are gentiy dispensed into 96- or 384-well plates and afterwards these
clones of inferest are expanded, giving rise fo manoclonal colonies. Further characterisafion analysis
can be done, i.e Genomics characterisafion.

Download Application Notes

For scientists by scientists, these notes highlight novel ways to optimize
your research with our products and solutions
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Product Datasheet

SamplesUncultured microbiota, cultured E.coli. and yeast, etc.
Cell size0.5 – 20 µm
SortingCell morphology or fluorescence
Droplet volume~50 pL
Substrate96, 384-well plates, PCR or single well for agar
Compatible plate heightUp to 27 mm (ex: low-profile deep-well plates)
Automated Offset Correction (AOC) for precise positioning of the single cells Bottom imaging using 5x objective lens  
Optical resolution 2.2 µm 
Nozzle imagingCamera CMOS 
Magnification 20x 
Dual-channel: Brightfield and fluorescence 
Fluorescence nozzle imaging Ex. 488 nm 
Em. 504 – 596 nm 
Processing timesSingle-cell dispensing 96-well plate: ~ 3 min 
Single-cell dispensing 384-well plate: ~ 8 min 
Cartridge EASY.ON 20 µm disposable to avoid cross-contamination
AutomationLid opening and closing 
Compatible with standard automation arm and gripper 
Compatible with third-party automation clients 
Embedded computerWindows
CompatibilityStandard biosafety cabinets class 2, and anaerobic chambers
Footprint635 x 400 x 286 mm
Weight40 kg 
CertificationCE, CB, UL (TÜV), RoHS
Thank you for your interest in our product. We understand that every business has unique needs, and our pricing plans are tailored to meet those needs. For a customized quote and to learn more about our pricing options, please fill out the quote request form. We would be happy to help you find the best plan for your needs and budget and answer any questions you may have. Thank you!

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CYTENA is a part of BICO. Our products aim to reduce the
organ shortage and speed up drug development by
providing accessible life science solutions that combine
biology and technology, fundamentally shifting the global healthcare industry.

Read more at BICO.com

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