Cell Line Development | Gene Therapy|F.SIGHT™ | F.SIGHT™ 2.0 | F.QUANT
F.SIGHT™ 2.0
Our new, highly efficient and gentle single-cell dispenser with fluorescent sorting. Streamline your workflows from monoclonal cell culture development for biopharmaceutical manufacturing, to single-cell assays for basic scientific research.
Building on the previous generation of single-cell dispensers, the F.SIGHT 2.0 has an innovative and ultrafast dual-camera system for simultaneous brightfield and fluorescent full resolution images.
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Key Features & Benefits
Find out how our product can enrich your research
Fast and accurate single-cell dispenser
Single cells are gently dispensed into a 96-well plate in under 2 minutes or into a 384-well plate in under 7.
Dual imaging system
Overlay both brightfield and fluorescent images for precise identification of your cells of interest. Both cameras can be controlled independently and the power of the built-in blue laser is adjustable. Combined, their high dynamic range visualizes even the weakest fluorophores.
No risk of contamination
Eliminate the risk of cross contamination with single-use EASY.ON cartridges.
Assurance of single-cell isolation
Capture nozzle images for every dispensing run to confirm that only single cells were dispensed on the plate.
Cell Focusing technology
Align cells at the center of the dispensing cartridge for even better handling and processing of delicate samples.
Gentle single-cell dispensing
Handle and isolate delicate cells of interest with our no-pressure dispensing, minimizing the stress inflicted on cells.
Product Details
Discover what you can do
Fast and precise dispensing
The F.SIGHT 2.0 isolates single-cells in either 96- or 384-well plates quickly, dispensing an entire 384-well plate in under 7 minutes. The system’s versatility enables a multitude of different cells to be fluorescently labelled and isolated.
Minimizing cell loss
Add an additional layer of optimization with Cell Focusing technology, which gently aligns cells at the center of the dispensing cartridge for superior cell detection. It offers significant improvements when working with rare cell types as this alignment ensures that cells are perfectly captured and measured, reducing cell loss and even increasing processing speeds.
Powerful software
Following CYTENA’s customer-oriented philosophy, the software has been designed for easy use and quick setup of individual fluorescence experiments. Additionally, the F.SIGHT 2.0’s software can provide full resolution brightfield and fluorescence images along with an overlayed image for monoclonality assurance. Everything is always recorded and saved for future analysis and assurance of clonality.
Automation ready
The F.SIGHT 2.0 can be easily integrated into automated workflows, making it the fastest single-cell dispenser fully compatible with robotic automation.
Product
Applications
Accelerate your research
Cell line development
Gene therapy and
viral vector production
Cell therapy and stem
cell-based research
CRISPR gene editing
Exosome manufacturing
Featured Workflow
With the FSIGHT 2.0’s fluorescet sorting capabilities, only single cells of interest are isolated
and dispensed for monoclonal expansion. After isolation, clones of interest are expanded
and, subsequently, the clones with the best yield are selected.
Download Application Notes
For scientists by scientists, these notes highlight novel ways to optimize
your research with our products and solutions
your research with our products and solutions
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Product Datasheet
| Samples | Eukaryotic cells |
| Cell size | 5 – 40 µm |
| Sorting | Cell morphology and fluorescence |
| Droplet volume | ~200 pL |
| Substrate | 96- and 384-well plates |
| Nozzle imaging | Camera CMOS Magnification 10x Dual-channel: Brightfield and fluorescence |
| Fluorescence nozzle imaging | Ex. 488 nm Em. 520 +/- 36 nm |
| Processing times | Single-cell dispensing 96-well plate: ~ 2 min Single-cell dispensing 384-well plate: ~ 8 min |
| Cartridge | EASY.ON 40 µm disposable cartridge to avoid cross-contamination |
| Automation | Lid opening and closing Compatible with standard automation arm and gripper Compatible with third-party automation clients |
| Embedded computer | Windows |
| Compatibility | Standard biosafety cabinets class 2 |
| Footprint | 635 x 400 x 282 mm |
| Weight | 40 kg |
| Certification | CE, CB, UL (TÜV), RoHS |
Thank you for your interest in our product. We understand that every business has unique needs, and our pricing plans are tailored to meet those needs. For a customized quote and to learn more about our pricing options, please fill out the quote request form. We would be happy to help you find the best plan for your needs and budget and answer any questions you may have. Thank you!
