Livecyte

Livecyte is a live-cell imaging system that produces exceptionally high contrast time-lapse videos using Phasefocus’s patented ptychographic quantitative phase imaging (QPI) technology for a range of label-free assays with or without up to seven channels of complementary fluorescence. Automated single-cell tracking algorithms enable users to automatically characterize growth, morphology and motility of large populations of cells in a 96-well plate assay format.

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Key Features & Benefits

Find out how our product can enrich your research

Quantitative Phase Imaging

High-content imaging of even sensitive cells in a non-invasive way.

Automated Segmentation & Tracking

Removes the need for manually tracking cells, saving time and improving reproducibility.

Real-time
Data Analysis

Results are ready to view at the end of the experiment.

Assay
Plate Formats

Up to 96 well plate.

No Expensive Consumables

No need for expensive custom plates or plate inserts.

Complementary Fluorescence

Up to seven fluorescence channels for substantial experimental flexibility.

Intuitive
Integrated Software

Software that’s so easy to use, people (almost!) don’t need training.

Large Fields of View

Up to 4mm² captures the behavior of many cells giving statistically significant analysis.

Full phenotypic Description of Cells

Proliferation, Motility, Morphology analysis, all from every experiment making lab time more productive.

Product Details

Discover what you can do
High-contrast
Label-free Imaging
  • Livecyte’s QPI technology enables the acquisition of high-contrast images without needing phototoxic illumination or fluorescence labels meaning even the most sensitive cells can be imaged in a non-invasive way.
Automated
Single-cell Tracking
  • State-of-the-art automated tracking algorithms can segment and track individual cells enabling users to monitor their behavior over extended periods of time; not just the behavior of the population average.
Powerful
Integrated Analytics
  • Livecyte’s Analyse software provides summarized Dashboards of many different types of behavior, from morphological to motile, in every experiment making laboratory time more productive.
Assay-Level Analysis
  • Large fields of view (up to 4mm²) in an assay relevant plate format (up to 96 well plate) and this means the behavior of many cells can be captured making results more statistically robust.
Explore Population
Heterogeneity
  • Classify cells based on any combination of measured parameters enabling the comparison of subpopulations and how their behavior changes with drug treatment.
Single-cell Lineage
  • Quick and easy correction of the automated tracking enables users to determine cell lineage. The properties of these cells can then be monitored over time and viewed in the context of subsequent or previous generations.

Product
Applications

Accelerate your research

Cell Proliferation and Growth

Cell Motility

Scratch Wound

Macrophage Immunology

Mitosis Assays

Featured Workflow

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Download Application Notes

For scientists by scientists, these notes highlight novel ways to optimize
your research with our products and solutions

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Product Datasheet

Imaging Modalities Brightfield; Ptychographic quantitative phase imaging; Fluorescence (up to 7 channels)
Compatible Plate Formats Standard 6, 12, 24, 48, 96 well plates (glass and high-grade plastic), 35mm dishes, slides (inc. Ibidi µ-slides)
Required Plate Inserts None
Objective Lens Options 4x; two from 10x, 20x or 40x (Plan N, Fluorite and UPLXAPO options available, air only)
Camera High sensitivity sCMOS (2048 x 2048 pixels; 6.5µm pixel size; 16 bit; 82% Peak QE)
XY Stage Automatic Linear-encoded ASI stage
Z Stage Automatic Olympus Z-positioner
Ptychographic QPI Illumination Integrated low-power laser diode (typically 30µW/mm2 at 650nm wavelength)
Fluorescence Illumination CoolLED pE-300 or CoolLED pE-4000 options available
Fluorescence Filters System compatible with up to 7 channels (in addition to QPI modality)
QPI Field of View Up to 2 x 2mm with 10x lens
Lateral Resolution Up to 0.4µm with a UPLXAPO 20x
QPI Capture Rate Up to one image per 13s
Environment Control Integrated stand-alone incubator controlling temperature, CO2, humidity and evaporation
IT Bespoke Acquisition PC, Analysis PC and Network Attached Storage (up to 40Tb) options
Software Acquire acquisition software, Cell Analysis Toolbox analysis software available
Space Requirements 1800 x 800 x 900 mm (W x D x H)
Weight ~65kg depending on configuration
Power Requirements Nominal 700 W
Gas Requirements Supply of 5% CO2 (in air) either from a cylinder with regulator or from mains supply via a CO2 mixer
Thank you for your interest in our product. We understand that every business has unique needs, and our pricing plans are tailored to meet those needs. For a customized quote and to learn more about our pricing options, please fill out the quote request form. We would be happy to help you find the best plan for your needs and budget and answer any questions you may have. Thank you!
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