Adrian Zambrano

Linkedin Facebook X-twitter Instagram Induced pluripotent stem cell (iPSC)-based allogeneic cell therapies are set to transform many fields of healthcare, especially regenerative medicine1. These therapies mean that donor-derived iPSCs can be modified and scaled up for use as “off-the-shelf” treatments for neurodegenerative, cardiovascular, and retinal diseases, to name just a few. Automation is also emerging […]

Linkedin Facebook X-twitter Instagram Induced pluripotent stem cells (iPSCs) are an incredibly powerful technology that continues to transform many facets of biomedicine. One particularly exciting area is the use of iPSC-derived cells as therapeutic agents. The development of allogeneic iPS cell-based therapies offers “off-the-shelf” therapies for regenerative medicine to treat conditions like cardiovascular disease, retinal degradation, and neurodegenerative

Linkedin Facebook X-twitter Instagram Optimizing Your Monoclonal Antibody Production Workflow Monoclonal antibody production is a multi-step process that involves developing cell lines, optimizing culture conditions, and ensuring stringent quality control to produce consistent batches of high-quality, high-yield monoclonal antibodies. The production of monoclonal antibodies is crucial due to their therapeutic applications across cancer, immunology, and

Linkedin Facebook X-twitter Instagram Hybridoma Technology: Quality Control and Best Practices Hybridoma technology was first described in 1975 by Georges Köhler and Cesar Milstein, who were jointly awarded the Nobel Prize in Physiology or Medicine in 1984 for their discovery (Köhler & Milstein, 1975; The Nobel Prize in Physiology or Medicine 1984, n.d.). It enables

Linkedin Facebook X-twitter Instagram Gene Editing in Cell Line Development: Cutting-Edge Approaches Advances in gene editing have revolutionized biomedical research and cell line development. New techniques are changing how we approach drug discovery and therapy production, from enhancing the stability and productivity of monoclonal cell lines to creating precise disease models1. Techniques like clustered regularly

Linkedin Facebook X-twitter Instagram Mammalian Cell Line Development: Applications and Common Challenges Mammalian cell lines are the cornerstone of translational and basic biomedical research. They provide a relatively cheap and manageable model for countless disease types and are invaluable for the production of many established and cutting-edge therapies1. With the advent of gene editing techniques,

Linkedin Facebook X-twitter Instagram Innovations and Applications of Single-Cell Seeding Advanced single-cell seeding processes continue to revolutionize biomedical research and therapeutic production by ensuring precision and purity. Single-cell seeding minimizes variability and ensures clonality, allowing it to support critical therapeutic areas such as regenerative medicine and oncology. Single-cell seeding accelerates advanced therapy development and high-throughput

Linkedin Facebook X-twitter Instagram Single-Cell Seeding: From Basics to Advanced Methods The single-cell seeding process differs across laboratories and institutions, and there are many ways to perform single-cell seeding depending on the resources and equipment available1. While these methods can produce clonally distinct populations growing in a single well, they come with varying degrees of

Linkedin Facebook X-twitter Instagram Enabled by low volume non-contact dispensing A group from the Karolinska Institute in Stockholm (SE) performed a genomic sequencing study of the coronavirus, was conducted using a method called CUTseq (Zhang X. et al. 2019), which is a versatile technique for preparing multiplex DNA sequencing libraries from low-input samples. The research

Linkedin Facebook X-twitter Instagram Enabled by single prokaryotic cell sequencing using the B.SIGHT™ single bacteria dispenser A recent study from the Karlsruhe Institute of Technology and IMTEK department at the University of Freiburg in Germany, conducted using our single-bacteria dispenser, the B.SIGHT™, led to the identification of microbial “dark matter,” or uncultured microorganisms, in a

Linkedin Facebook X-twitter Instagram NEW PROTOCOL FOR FASTER AND MORE RELIABLE RNA COUNTING Postdoctoral researcher Christoph Ziegenhain has developed a new protocol for faster and more reliable RNA counting. Based at the Sandberg Lab at the Karolinska Institute in Stockholm, Sweden, Ziegenhain focuses primarily on single-cell quantitative transcriptomics methods. His latest paper, published in Nature

Linkedin Facebook X-twitter Instagram GENETICALLY ENGINEERED SINGLE-CELL CLONES Characterizing CRISPR/Cas9-engineered cells is a major priority for pharmaceutical companies, as well as for novel cellular therapeutic applications and regenerative medical uses. For this reason, the team at the Department of Microsystems Engineering at the University of Freiburg, under Dr. Stefan Zimmermann’s leadership, developed a new workflow