B.SIGHT™

Label-free single-cell isolation of bacteria and other microorganisms

Dispense bacteria, yeast and archaea using single-cell technology

It is now possible to isolate individual prokaryotic cells using CYTENA’s validated single-cell dispensing technology. The B.SIGHT’s extremely high-resolution optics make even the smallest cells visible. No staining or labeling is required to isolate single bacterium.

B.SIGHT™

Single bacteria dispensing using single-cell technology

Ideal for Anaerobic Conditions

The benchtop B.SIGHT can be operated in an anaerobic chamber thanks to its small footprint, low particle emission and low heat emission. These characteristics provide the ideal environment for isolating obligate anaerobes. You can also control the device from outside the cabinet by connecting a monitor, keyboard and mouse, or by using a remote control terminal.

Start sorting right away

Isolating cells is simple and contamination free with our disposable cartridges. Simply pipette your sample into the reservoir and mount the cartridge to the B.SIGHT. Microscopic droplets are produced through our bacteria dispensing cartridges to create stable and accurate bacteria encapsulation.

ISOLATE FLUORESCENT MICROORGANISMS

CYTENA offers customers the option to add one integrated laser for fluorescent cell sorting. Choose the laser that best suits your needs and start sorting right away.

Intuitive and easy to use software

Use the interactive well plate to design your own unique dispensing experiment and see in real time how each bacterium is sorted through the nozzle of the cartridge and dispensed into your 96- or 384-well plate. Images of the dispensed single bacterium are stored on the hard drive, providing proof of clonality.

MICROBIOLOGY RESEARCH MADE EASY

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High-resolution optics

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Automation ready

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Compatible with anaerobic conditions

ISOLATE MORE SPECIES WITH THE B.SIGHT

List of pure cultures isolated with the B.SIGHT

  • Bacillus subtilis
  • Bacillus vulgatus (strict anaerobe)
  • Enterococcus faecalis
  • Escherichia coli (Top10, DH5alpha, BL21, etc.)
  • Staphylococcus aureus
  • Various cyanobacteria strains
  • Saccharomyces cerevisiae (yeast)
  • Pichia pastoris (yeast)

List of complex samples processed with the B.SIGHT

  • Human faecal samples
  • Mouse caecal samples
  • Human oral microbiome
  • Wastewater
  • Marine samples

RELEVANT APPLICATION NOTES

B.SIGHT – Accelerating bacteria transformation and cloning workflows using microbial single-cell isolation

B.SIGHT – High-throughput Cultivation Workflow for Isolation of Anaerobic Gut Bacteria

B.SIGHT – Sequencing Single-cell Genomes of Uncultivated Bacteria in the Human Oral Microbiome

Control the device from outside your anaerobic bench

Simply connect the monitor, keyboard and mouse or even use a remote control terminal

READY TO RETHINK MICROBIOLOGY?

 Contact us at [email protected].