C.LIVE Caspase-3
Green NucView®

The C.LIVE Caspase-3 assay is a standardized, fluorescence-based assay that measures caspase-3 protease activity for apoptosis detection. This reagent contains a cell-permeable dye that is coupled to the caspase-3 substrate peptide sequence DEVD blocking its binding to DNA. If activated in apoptotic cells, caspase-3 cleaves the peptide sequence, and the dye is released to bind to the DNA with high-affinity, resulting in a fluorescent signal in the cell nucleus.

This allows for early detection of cell death independent of morphology changes of the cells. With one vial, you can analyze 400 samples (with 100 µL per sample) which is equal to 4 full 96-well plates of experiments. Together with the power
of the CELLCYTE X and C.LIVE reagents, open
the door to live cell imaging and view the
biological events in your cell samples with ease

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Key Features & Benefits

Find out how our product can enrich your research

Direct detection of activation of
caspase-3 as early indicator of
programmed cell death

Experience real-time detection of apoptosis inside the incubator.

Mix-and-read workflow

Seed, treat, and image your cells in three easy steps.

Optimized for live cell imaging analysis

Observe cell health behavior instantly without hinderance from optimal assay conditions.

Cell health integrity upheld

Confidently analyze your samples with the assurance that our reagent does not impair cell growth.

Product Details

Discover what you can doDirect, Real-time Detection of
Apoptosis Inside the Incubator
In the figure below, HeLa cells were treated with increasing concentrations of Staurosporine. C.LIVE Caspase-3 was added to each well (2.5 µM) and timelapse images were obtained with the CELLCYTE X. The DMSO control well shows minimal green signal compared to the Staurosporine-treated well at 48 hours (top panel). Automated quantification shows the time- and dose-dependent cytotoxic effects of Staurosporine (bottom panel).

Confidence in your cell
health integrityAs shown below, our caspase reagent does not impair cell growth, which gives you the assurance that your cell health integrity will remain upheld during the experiment. Whether you’re analyzing cancer cell health, drug screening, immune cell killing, or early toxicity, the combination of C.LIVE Caspase and the CELLCYTE X can handle any application.

Product
Applications

Accelerate your research

Apoptosis

Immune cell health

Immune cell growth

Immune cell differentiation

Immuno-oncology

Featured Workflow

Run your apoptosis assays in three simple steps shown below.

Download Application Notes

For scientists by scientists, these notes highlight novel ways to optimize
your research with our products and solutions

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Product Datasheet

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Product Name	Price	Channel	Amount	Concentration	Samples	Storage	Stability
C.LIVE Caspase-3 Green NucView®	$340	Green	20 µL	5 mM (2000X stock solution)	400	-20°C	12 months

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