F.SIGHT™ 2.0

Our new, highly efficient and gentle single-cell dispenser with fluorescent sorting. Streamline your workflows from monoclonal cell culture development for biopharmaceutical manufacturing, to single-cell assays for basic scientific research.

Building on the previous generation of single-cell dispensers, the F.SIGHT 2.0 has an innovative and ultrafast dual-camera system for simultaneous brightfield and fluorescent full resolution images.

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Key Features & Benefits

Find out how our product can enrich your research

Fast and accurate single-cell dispenser

Single cells are gently dispensed into a 96-well plate in under 2 minutes or into a 384-well plate in under 7.

Dual imaging system

Overlay both brightfield and fluorescent images for precise identification of your cells of interest. Both cameras can be controlled independently and the power of the built-in blue laser is adjustable. Combined, their high dynamic range visualizes even the weakest fluorophores.

No risk of contamination

Eliminate the risk of cross contamination with single-use EASY.ON cartridges.

Assurance of single-cell isolation

Capture nozzle images for every dispensing run to confirm that only single cells were dispensed on the plate.

Cell Focusing technology

Align cells at the center of the dispensing cartridge for even better handling and processing of delicate samples.

Gentle single-cell dispensing

Handle and isolate delicate cells of interest with our no-pressure dispensing, minimizing the stress inflicted on cells.

Product Details

Discover what you can do

Fast and precise dispensing

The F.SIGHT 2.0 isolates single-cells in either 96- or 384-well plates quickly, dispensing an entire 384-well plate in under 7 minutes. The system’s versatility enables a multitude of different cells to be fluorescently labelled and isolated.
Minimizing cell loss
Add an additional layer of optimization with Cell Focusing technology, which gently aligns cells at the center of the dispensing cartridge for superior cell detection. It offers significant improvements when working with rare cell types as this alignment ensures that cells are perfectly captured and measured, reducing cell loss and even increasing processing speeds.
Powerful software

Following CYTENA’s customer-oriented philosophy, the software has been designed for easy use and quick setup of individual fluorescence experiments. Additionally, the F.SIGHT 2.0’s software can provide full resolution brightfield and fluorescence images along with an overlayed image for monoclonality assurance. Everything is always recorded and saved for future analysis and assurance of clonality.

Automation ready
The F.SIGHT 2.0 can be easily integrated into automated workflows, making it the fastest single-cell dispenser fully compatible with robotic automation.


Accelerate your research

Cell line development

Gene therapy and
viral vector production

Cell therapy and stem
cell-based research

CRISPR gene editing

Exosome manufacturing

Featured Workflow

With the FSIGHT 2.0’s fluorescet sorting capabilities, only single cells of interest are isolated and dispensed for monoclonal expansion. After isolation, clones of interest are expanded and, subsequently, the clones with the best yield are selected.

Download Application Notes

For scientists by scientists, these notes highlight novel ways to optimize
your research with our products and solutions
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Product Datasheet

SamplesEukaryotic cells
Cell size5 – 40 µm
SortingCell morphology and fluorescence
Droplet volume~200 pL
Substrate96- and 384-well plates
Nozzle imaging

Camera CMOS

Magnification 10x

Dual-channel: Brightfield and fluorescence

Fluorescence nozzle imaging

Ex. 488 nm

Em. 520 +/- 36 nm

Processing times

Single-cell dispensing 96-well plate: ~ 2 min

Single-cell dispensing 384-well plate: ~ 8 min

CartridgeEASY.ON 40 µm disposable cartridge to avoid cross-contamination
AutomationLid opening and closing
Compatible with standard automation arm and gripper
Compatible with third-party automation clients
Embedded computerWindows
CompatibilityStandard biosafety cabinets class 2
Footprint635 x 400 x 286 mm
Weight40 kg
CertificationCE, CB, UL (TÜV), RoHS
Thank you for your interest in our product. We understand that every business has unique needs, and our pricing plans are tailored to meet those needs. For a customized quote and to learn more about our pricing options, please fill out the quote request form. We would be happy to help you find the best plan for your needs and budget and answer any questions you may have. Thank you!

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