Single cell RNA-seq with ultra-high sensitivity and a streamlined workflow
The new plexWell Rapid Single Cell RNA Library Prep Kit uses an optimised chemistry for highly sensitive full-length cDNA synthesis. It captures the complexity of the transcriptome, including splice variants, fusion transcripts and SNPs.
The innovative tagmentation-based library preparation reduces hand-on time, removes the need for sample normalization and allows for highly multiplexed sequencing.
The plexWell Rapid Single Cell RNA Library Prep Kit was benchmarked against common Smart-seq protocols (1,2) using a challenging sample with ultra-low cellular RNA content.
The plexWell Kit achieved the highest sensitivity (Fig.1). Furthermore, the plexWell Kit delivered a very uniform gene body coverage, the highest percentage of uniquely mapping reads, the highest exon- and the lowest intron-mapping rate.
The plexWell Rapid Single Cell RNA Library Prep Kit facilitates library preparation with a unique barcoding scheme and built-in automatic normalization, reducing the need for sample QC. The whole process from sorted cells to sequencing-ready libraries can be completed in the same day, without any specialized equipment.
plexWell auto-normalization ensures a highly even read distribution across single cells. Different sets of barcodes are available for multiplexing up to 1152 cells in one sequencing run.
- Picelli et al., Nature Protocols 9(1):171-81 (2014)
- Hagermann-Jenssen et al., Nature Biotechnology 38(6):708-714 (2020)