plexWell’s integrated normalizing library preparation technology allows the creation of balanced library pools without the need for sample or library normalization. The simplified 3-hour workflow multiplexes 100 to 1000 samples for loading in a single sequencing run, resulting in better overall sequencing performance.Multiplex 1000’s of samples with ease.Built-in normalization with every kit.Uniform insert sizes & sample read counts.Improved overall sequencing performance.
Unfragmented DNA is tagged with sample-specific i7-barcoded adapters.
P7 primers and unique i7 indexes are inserted randomly into each DNA sample by a transposon. The same amount of P7 is added to each sample regardless of DNA input amount. This limiting reagent normalizes the samples, which are now pooled into a single tube.
Pooled sample-barcoded DNA is tagged with pool-specific i5-barcoded adapters.
The pool-specific i5 barcoded adapters containing P5-adapters are now added in the second transposition reaction. Excess pool barcoding reagent inserts the same average distance from every sample barcode.
Barcoded library fragments are amplified.
The final step is a library amplification with universal primers and final SPRI purification. The pooled library is now ready to be loaded in an Illumina® sequencer to produce normalized or balanced distribution of sequencing reads.The technical foundation of the plexWell approach is an initial reagent-limited tagging step performed on many samples in parallel, coupled with a subsequent pooled library generation step. When applied together, these two steps produce an approximately equal number of sequenceable library fragments from each of a potentially large collection of samples. The two-step process also allows for a large combinatorial repertoire of barcode combinations.Not available in the US, Canada, Australia and New Zealand.Simple and scalable multiplexed workflow for 1000’s of samples.Premier single cell RNA Sequencing.