B.SIGHT
Unmatched Microbiome Isolation Meets Unrivaled Research Experience.
- Dual-channel high resolution microscopy
- High-speed isolation of cells
- Assurance of clonality
- High cell viability
- Compatible with anaerobic conditions
- Single-cell genomics of uncultivated bacteria
- Automation ready
Trusted By 1000s Of Your Peers At Premier Research Labs Worldwide
This does not happen by accident.
Our Precision Engineering Meets Your Breakthrough Research
Key Features & Benefits
Find out how our product can enrich your research
Automation ready
The B.SIGHT automatically releases the multiwell plate after dispensing is complete and automatically opens up the lid, thereby allowing robotic arms to transfer the plate downstream for further processing inside an automated workstation.
Ideal for anaerobic conditions
The B.SIGHT can be operated in an anaerobic chamber thanks to its small footprint, low particle emission and low heat emission. These characteristics provide the ideal environment for isolating strict anaerobes. You can also control the device from outside the cabinet by connecting a monitor, keyboard and mouse, or by using a remote control terminal.
Unprecedented dispensing speed
Isolation of bacteria into multi-well plates is completed in minutes. The B.SIGHT takes only 3 minutes to complete the isolation of bacteria into a 96-well plate and 8 minutes into 384-well plate!
Dual-camera system
The B.SIGHT’s extremely high-resolution optics make even the smallest cells visible. With an innovative and ultrafast dual-camera system, the system can efficiently and rapidly separate cultures of both fluorescent and wild-type bacteria into either liquid culture or agar.
Isolate fluorescent microorganisms
With the B.SIGHT, you can easily isolate fluorescent-labelled cells in minutes.
Specialized for OMICS
The B.SIGHT enables single-cell genomics of microbiome samples without the need for cultivation. The B.SIGHT’s Automated Offset Correction (AOC) allows precise and accurate dispensing of single cells encapsulated in picolitre droplets at the center of wells in PCR plates, thus enabling downstream miniaturization of lysis buffers for NGS library preparation.
Avoid cross contamination:
Isolating cells is simple and contamination free with our disposable EASY.ON cartridges. Simply pipette your sample into the reservoir and mount the cartridge to the B.SIGHT. Microscopic droplets are produced through our bacteria-dispensing cartridges to create stable and accurate bacteria encapsulation. A new sample is then loaded into a new cartridge without a possibility of cross contamination.
Intuitive and easy-to-use software
Use the interactive well plate to design your own unique dispensing experiment and see in real time how each bacterium is sorted through the nozzle of the cartridge and dispensed into 96- or 384-well plates. Images of the dispensed single bacterium are stored on the hard drive, providing proof of clonality.
Wide range of target plates
The device can host a wide range of multiwell plates (96-, 384- and single-well plates) with heights up to 27 mm, enabling the use of low-profile deep well plates.
Isolate more species with the B.SIGHT
Enabling scientists worldwide
Dual-channel high resolution microscopy
Detects even the smallest cells based on size and shape. The system comes with full capability to isolate unlabeled and fluorescently labeled single microorganisms.
High-speed isolation of cells
Accelerate your microorganism workflows with high-speed isolation of single bacteria or yeast into multiwell plates. Process a 96-well plate in 3 minutes and a 384- plate in under 8 minutes.
Assurance of clonality
Nozzle images confirm the clonality of dispensed single prokaryotic cells.
High cell viability
The B.SIGHT improves cell viability thanks to fast, gentle and easy-to-use label-free isolation of single bacteria directly into liquid culture or agar.
Compatible with anaerobic conditions
Small benchtop instrument that integrates into fume hoods and anaerobic chambers. Simply connect the monitor, keyboard and mouse or even use a remote control terminal.
Single-cell genomics of uncultivated bacteria
Dispense single cells from your complex microbiota sample in picolitre droplets directly into the center of the well in 96- or 384-well PCR plates, enabling downstream miniaturization with lysis buffer volumes less than 1 μL.
Automation ready
The B.SIGHT lid opens automatically, making the device compatible with robotic automation workflows. The instrument also has a small footprint and can easily be placed in most biosafety cabinets.
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List of pure cultures isolated with the B.SIGHT
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- Bacillus subtilis
- Bacillus vulgatus (strict anaerobe)
- Enterococcus faecalis
- Escherichia coli (Top10, DH5alpha, BL21, etc.)
- Staphylococcus aureus
- Various cyanobacteria strains
- Acetobacter
- Pseudomonas
- Acinetobacter baumannii
- Septoria cytisi(fungi)
- Saccharomyces cerevisiae (yeast)
- Pichia pastoris (yeast)
- List of complex samples processed with the B.SIGHT
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- Human faecal samples
- Mouse caecal samples
- Human oral microbiome
- Wastewater
- Marine samples
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Impact Areas - Use Cases
Cell Line Development
Product Resources
Application Notes
Peerless: In Quality and
Customer Support
Global
Market Leader
Pioneers of the single-cell dispensing category
Made
in Germany
Engineered with Uncompromising Quality
Onsite Service
& Support
Europe, UK, USA, Singapore, South Korea and Japan
World
Class Expertise
Years of History of Success and Growth
