Single-cell protein quantification is key for exploring cellular heterogeneity but often lacks sensitivity, throughput, or accuracy. The scPICO assay was developed to enable reference-free absolute quantification of proteins at the single-cell level. It combines the F.SIGHT for precise single-cell dispensing and the I.DOT for ultra-low-volume reagent handling.
The resulting workflow delivers highly sensitive, high-throughput single-cell proteoform analysis with minimal background interference.
Learn more about how:
The F.SIGHT isolates single cells into 384-well plates with sub-nanoliter precision
The I.DOT performs low-volume dispensing of labeled antibodies in the scPICO workflow
scPICO enables absolute quantification of proteins and phosphorylation states at single-cell resolution, revealing cellular heterogeneity
