Producer’s cell lines must be derive from a single-cell progenitor to ensure consistency, quality, and safety of a therapeutic product. In this technote, we introduce two innovative capabilities of the UP.SIGHT™. Automated Offset Correction (AOC), which ensures dispensed single cells land near the center of the well, and 3D Well Imaging, which capture images of the […]
The UP.SIGHT enables gentle, high-recovery single-cell cloning of human iPSCs with built-in imaging-based verification of clonality. Through real-time nozzle inspection and 3D full-well imaging, the system ensures monoclonality with over 99.99% accuracy. Optimized dispensing conditions, such as saline buffer and enhanced cloning media, boosted clone recovery up to 80%, while ultra-fast automated plate imaging enabled efficient
CRISPR/Cas9 editing enables precise modifications in mesenchymal stem cells (MSCs), but isolating monoclonal clones remains a bottleneck. In this study, the F.SIGHT™ enabled gentle, fluorescence-guided single-cell isolation of MSCs edited to knock out RANKL, even when expression levels were low. With over 93% single-cell isolation efficiency, the system generated 182 viable clones – ultimately yielding
Ensuring monoclonality in cell line development is essential yet challenging. In this study, the UP.SIGHT™ combines double verification of single-cell dispensing, via nozzle imaging and novel 3D full-well imaging, to confirm monoclonality (>99.99%) without centrifugation or fluorescent labeling. Compatible with 96-, 384-, and 1536-well plates, the UP.SIGHT enables high-throughput single-cell cloning and colony tracking, with minimal
Understanding cell proliferation is essential across both basic and translational research, where pathological changes often manifest in altered growth patterns. The CELLCYTE X live-cell imaging system was applied to monitor proliferation in multi-well plates, delivering a comprehensive and real-time overview of cell growth. By combining Enhanced Contour-based confluence with fluorescence intensity and cell counting, the
CELLCYTE X™ – Real-time Multiparametric Pharmacological Screening with Live Cell Imaging Read More »
CYTENA is a part of BICO. BICO is a lab automation partner and provider of selected workflows to pharma and biotech. Operating through two business areas – Lab Automation and Life Science Solutions – BICO strives towards the vision to enable and automate the life science lab of the future.
Read more at BICO.com
⚡Discover the C.STATION™ – Combine Single-Cell Cloning, Upscaling And Clone Selection All In One Unit. See How.
