Producer’s cell lines must be derive from a single-cell progenitor to ensure consistency, quality, and safety of a therapeutic product. In this technote, we introduce two innovative capabilities of the UP.SIGHT™. Automated Offset Correction (AOC), which ensures dispensed single cells land near the center of the well, and 3D Well Imaging, which capture images of the […]
Efficient and reproducible washing is essential for immunoassay sensitivity and consistency. To optimize beyond manual and semi-automated methods, the LUNARIS multiplex assay protocol was adapted to the C.WASH washing and liquid handling system. This technology enables straightforward use with 384-well plates, processing large sample numbers without compromising volume, sensitivity, or robustness – while reducing hands-on
The UP.SIGHT enables gentle, high-recovery single-cell cloning of human iPSCs with built-in imaging-based verification of clonality. Through real-time nozzle inspection and 3D full-well imaging, the system ensures monoclonality with over 99.99% accuracy. Optimized dispensing conditions, such as saline buffer and enhanced cloning media, boosted clone recovery up to 80%, while ultra-fast automated plate imaging enabled efficient
Fluorescence-based workflows enhance single-cell cloning efficiency and clonality assurance. In this study, the F.SIGHT was used to isolate fluorescently stained cells, enabling clear viability assessment and direct correlation of GFP expression with gene-of-interest production. Our findings support the use of the F.SIGHT as a reliable platform for fluorescence intensity–based single-cell isolation in CLD workflows. Learn
CRISPR/Cas9 editing enables precise modifications in mesenchymal stem cells (MSCs), but isolating monoclonal clones remains a bottleneck. In this study, the F.SIGHT™ enabled gentle, fluorescence-guided single-cell isolation of MSCs edited to knock out RANKL, even when expression levels were low. With over 93% single-cell isolation efficiency, the system generated 182 viable clones – ultimately yielding
Ensuring monoclonality in cell line development is essential yet challenging. In this study, the UP.SIGHT™ combines double verification of single-cell dispensing, via nozzle imaging and novel 3D full-well imaging, to confirm monoclonality (>99.99%) without centrifugation or fluorescent labeling. Compatible with 96-, 384-, and 1536-well plates, the UP.SIGHT enables high-throughput single-cell cloning and colony tracking, with minimal
Acoustophoresis-based cell focusing improves precision in single-cell applications by aligning cells prior to dispensing. In this study, the UP.SIGHT™ was used to enhance cell isolation efficiency, recovery rate, and dispensing speed at low concentrations while maintaining high cell viability. Our findings support the use of the UP.SIGHT as a reliable platform for accurate, fast, and gentle
Acoustophoresis-based Cell Focusing Enables Enhanced Single-cell Dispensing Read More »
Suspension cells are essential in immunology and hematology, yet conventional centrifugation and manual washing can cause variability and cell loss. The C.WASH was evaluated for washing suspension cells in 96-well microtiter plates for phenotypic characterization by flow cytometry. Using centrifugal forces and contact-free dispensing, the system gently removed medium while retaining pelleted cells. Compared to manual
C.WASH™ – Automated noncontact washing of suspension cells Read More »
The F.SIGHT was used to isolate and verify monoclonal single-cell clones from pools of differently barcoded subpopulations. This process enables deconvolution of complex cell populations to directly connect genetic alterations with their biological effects, without compromising clonal diversity. With its precision and reliability, the F.SIGHT proved crucial for achieving accurate single-cell isolation in this workflow. Learn
F.SIGHT™ – Deconvoluting clonal complexity of barcoded cell populations Read More »
CYTENA is a part of BICO. BICO is a lab automation partner and provider of selected workflows to pharma and biotech. Operating through two business areas – Lab Automation and Life Science Solutions – BICO strives towards the vision to enable and automate the life science lab of the future.
Read more at BICO.com
⚡Discover the C.STATION™ – Combine Single-Cell Cloning, Upscaling And Clone Selection All In One Unit. See How.
