Combined Single-cell Dispensing and 3D Full Well Imaging for Cell Lines with >99.99% Probability of Monoclonality

Ensuring monoclonality in cell line development is essential yet challenging. In this study, the UP.SIGHT combines double verification of single-cell dispensing, via nozzle imaging and novel 3D full-well imaging, to confirm monoclonality (>99.99%) without centrifugation or fluorescent labeling. Compatible with 96-, 384-, and 1536-well plates, the UP.SIGHT enables high-throughput single-cell cloning and colony tracking, with minimal hands-on time and increased efficiency.

Our findings support the UP.SIGHT as an all-in-one solution for rapid, accurate, and verifiable single-cell cloning workflows.

Learn more about how:

• The UP.SIGHT provides dual assurance of monoclonality with nozzle and 3D full-well imaging
• Imaging the full liquid volume eliminates the need for centrifugation and prevents ghost well artifacts
• Compatibility with multiple plate formats enables high-throughput cloning and colony tracking