ELISA

Automate and optimize your ELISAs for faster and more efficient screening.

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Automating ELISA Workflows

Enzyme-linked immunosorbent assays (ELISAs) are widely used to quantify antigens, antibodies, and proteins across research and diagnostic applications. Detection relies on antibodies immobilized on a solid phase, where an enzymatic reaction generates a measurable signal. Removing non-specifically bound proteins is essential for minimizing background signal and ensuring reliable results.

ELISA workflows typically require multiple wash cycles after each reaction step. CYTENA's C.WASH Plus uses centrifugal force for contact-free plate washing, reducing the time and consumables required for washing while improving consistency and minimizing sample disturbance.

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A Typical ELISA Workflow

Explore how CYTENA solutions support and automate key stages of a sandwich ELISA workflow, from antibody binding and reagent addition to efficient plate washing and signal development.

 

1. Plate Coating

Immobilize capture antibodies onto the microplate surface to prepare for antigen binding in subsequent steps.

2. Capture Antibody Dispensing

Dispense precise capture antibody volumes across the plate with non-contact liquid handling for uniform, reproducible coating.

Solution: I.DOT

3. Blocking & Washing

Block unbound sites and remove excess reagent using centrifugal, contact-free washing to minimize background noise.

Solution: C.WASH Plus

4. Sample & Antigen Incubation

Add samples and standards with accurate, low-volume dispensing to ensure consistent antigen binding across the plate.

Solution: I.DOT

5. Detection Antibody & Substrate Washing

Wash between detection antibody and substrate addition steps in as few as two cycles, preserving signal integrity.

Solution: C.WASH Plus

6. Signal Detection & Analysis

Measure the enzymatic readout and quantify antigen concentration for confident, reproducible results.

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High-Throughput ELISA Screening

Automate critical ELISA workflow steps with precision dispensing and contact-free plate washing. The combination of I.DOT and C.WASH Plus enables scalable, reproducible screening workflows from assay development to high-throughput execution.

 Solutions: I.DOT + C.WASH PLUS

Diagnostic & Biomarker Assays

Improve assay sensitivity and reproducibility through efficient, contact-free washing. C.WASH PLUS minimizes residual liquid and background interference, helping deliver robust and reliable biomarker assay results.

Solutions: C.WASH Plus

Centrifugal Washing LR

Application Notes

APPLICATION NOTE

Improved ELISA Workflow Efficiency Using Automated Plate Washing

Streamlined ELISA processing using automated non-contact washing to boost assay throughput and eliminate manual pipetting steps.

 

 

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APPLICATION NOTE

C.WASH PLUS Plate Washer Allows Reusing of ARTEL MVS Microplates

High-precision microplate washing that cleans and enables the reuse of verification plates without compromising optical accuracy.

 

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APPLICATION NOTE

Highly Efficient Washing of Microwell Plates Using Centrifugal Forces

Rapid non-contact centrifugal washing that removes supernatants and cuts background noise while maximizing immunoassay sensitivity.

 

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APPLICATION NOTE

Multiplex Protein Analysis Using LUNARIS™ Kits and CYTENA C.WASH 

Automated non-contact washing for multiplex immunoassays to lower %CV variability, cut reagent waste, and maximize signal clarity.

 

 

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Solutions for ELISA

C.WASH PLUS

Centrifugal plate washing system, compatible for 96-, 384-, and 1536-well plates.

I.DOT

Tipless, low-volume non-contact dispensing with the I.DOT for fast, precise liquid handling with minimal waste across life science applications.

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Frequently Asked Questions

Why is contact-free plate washing beneficial for ELISA assays?

Contact-free centrifugal washing minimizes disturbance of assay surfaces while efficiently removing residual liquids. This helps reduce background noise, improve signal-to-noise ratios, and support reliable, reproducible ELISA results across a wide range of applications.

How can I increase ELISA throughput without compromising data quality?

Combining precise reagent dispensing with rapid plate washing enables laboratories to process more samples while maintaining assay consistency.
Solutions such as I.DOT and C.WASH PLUS support scalable ELISA workflows with reduced hands-on time and reproducible performance.

Which ELISA workflows can be automated with CYTENA solutions?

CYTENA solutions support a variety of ELISA workflows, from assay development and optimization to routine screening and biomarker studies. Automated dispensing and washing help streamline multi-step protocols while improving consistency between plates and operators.

How does C.WASH PLUS help improve ELISA data quality?

C.WASH PLUS achieves efficient liquid removal with minimal residual volume, helping reduce background interference and contributing to more consistent assay results.