INTRODUCING
C.LIVE CASPASE-3 GREEN NUCVIEW®

Rapidly Detect Apoptotic Cells Inside Your Incubator

THE SCIENCE BEHIND THE PRODUCT

The C.LIVE Caspase-3 assay is a standardized, fluorescence-based assay that measures caspase-3 protease activity for apoptosis detection. This reagent contains a cell-permeable dye that is coupled to the caspase-3 substrate peptide sequence DEVD blocking its binding to DNA. If activated in apoptotic cells, caspase-3 cleaves the peptide sequence, and the dye is released to bind to the DNA with high-affinity, resulting in a fluorescent signal in the cell nucleus. This allows for early detection of cell death independent of morphology changes of the cells.

ADVANTAGES OF CYTENA APOPTOSIS ASSAYS

1

Direct detection of activation of caspase-3
as early indicator of programmed cell death

2

Mik-and-read workflow

3

Optmized for live cell imaging analysis

4

Cell health integrity upheld

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HeLa cells were treated with increasing concentrations of Staurosporine. C.LIVE Caspase-3 was added to each well (250 nM)  and timelapse images were obtained with the CELLCYTE X. The control well shows minimal green signal compared to Staurosporine-treated well at 48 hours (top). Automated quantification shows the time- and dose-dependent toxicity effects of Staurosporine (bottom).

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MIX-AND-READ WORKFLOW

Optimize and streamline every step of your
caspase assay workflow to fit your protocol in
three simple steps shown on the left.

Direct, real-time detection of apoptosis inside the incubator

Use the CELLCYTE X to image and
quantify fluorescent signals of your
caspase assay without removing
the cells from the incubator.

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Confidence in Your Cell Health Integrity

The substrate does not bind to DNA, however, once enzyme cleavage occurs, the high-affinity DNA dye is released to bind to the DNA to fluoresce the nucleus. This allows for early detection of cell death with or without morphology changes inside your incubator.

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As shown, our caspase reagent does not impair cell growth, which gives you the assurance that your cell health integrity will remain upheld during the staining process.

Related products

C.LIVE Caspase Reagent Product Details

Product Name Price Channel Amount Concentration Samples Storage Stability
C.LIVE Caspase-3 Green NucView® $340 Green 20 µL 5 mM (2000X stock solution) 400 -20°C 12 months

CELLCYTEX

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C.LIVE