F.QUANT Titer

HIGH-THROUGHPUT TITER QUANTIFICATION ASSAYS FOR ANTIBODIES, BISPECIFICS AND FC FUSION PROTEINS

Titer assays measure the concentration of target antibodies in samples (e.g., serum, plasma and culture supernatant). Fast and reproducible titer measurement is essential for the identification of high producer cell lines for manufacturing biotherapeutics and enabling high-throughput antibody discovery workflows.

The F.QUANT Titer assay kits, Powered by PAIA, are designed for rapid quantification of antibody concentration in culture supernatant and based on PAIA technology. The F.QUANT Fc Titer is specific for Fc in IgG and other proteins containing the Fc part of IgG, such as Fc fusion proteins and bispecifics. The F.QUANT Fab Titer assay recognizes human Fab fragments.

Increasing concentrations of Trastuzumab (0 – 1000 µg/ml) are measured as duplicates using F.QUANT Fc Titer assay. Signal intensities were detected using fluorescence microplate reader.

MIX, SHAKE AND READ PROTOCOL FOR TITER QUANTIFICATION ASSAYS

Run your titer quantification assays in simple steps:

Combine the F.QUANT Titer Mix with a supernatant in the F.QUANT Titer Plate, shake the plate and read on a fluorescence microplate reader.

ADVANTAGES OF CYTENA’S TITER ASSAYS

Direct titer quantification from supernatant

No cell removal or wash necessary

Optimized for clone screening in CLD

Easy mix, shake and read workflow

Simple automation on liquid handlers

Assays require as little as 5 µL supernatant

KIT CONTENTS

Each kit includes a 384-well F.QUANT Titer Plate containing desiccated capture beads and a bottle of fluorescent F.QUANT Titer Mix.

SPECIFICATIONS

  • Solution-based detection
  • Fluorescence microplate reader
  • Excitation wavelength: 640 nm; Emission wavelength: 665 nm

F.QUANT Titer Assay Kits Product Information

Product Name ID Amount Content Storage Stability
F.QUANT Fc Titer CY.FQ.TIT.103 10 plates/3840 samples 10x 384-well F.QUANT Fc Titer Plate and 200 mL Fc Titer Mix 4 °C 6 months
F.QUANT Fab Titer CY.FQ.TIT.104 10 plates/3840 samples 10x 384-well F.QUANT Fab Titer Plate and 200 mL Fab Titer Mix 4 °C 6 months
F.QUANT Fc Titer
*Trial size
CY.FQ.TIT.101 2 plates/768 samples 2x 384-well F.QUANT Fc Titer Plate and 2x 25 mL Fc Titer Mix 4 °C 6 months
F.QUANT Fab Titer
*Trial size
CY.FQ.TIT.102 2 plates/768 samples 2x 384-well F.QUANT Fab Titer Plate and 2x 25 mL Fab Titer Mix 4 °C 6 months

READY TO ACCELERATE YOUR CLD WORKFLOW?