microbiome research

microbiome research

A critical step in the process of isolating and studying microbes from complex communities is efficient separation of single community members for obtaining pure cultures. This is complicated by the uneven distribution and high complexity of mixed microbial samples such those from human feces, or from environmental sources like marine samples or waste water.

Traditionally this is done by colony streaking on agar plates or dilution-to-extinction. Using these methods, it is often difficult to obtain pure cultures due to the unknown composition and heterogeneous cell replication rates. Therefore we developed the b.sight, which enables automated and label-free single-cell isolation from complex microbial samples.

Individual cells are directly isolated into liquid culture or agar. The small footprint and simple handling of the instrument allows easy integration into an anaerobic workflow.

typical work flow

Sample preparation

Prepare a single-cell suspension from your microbiome sample

Single-cell isolation

Isolate your cells without bias directly into liquid growth media.

Cultivation

Incubate the isolated single-cells to obtain pure clonal cultures.

Monitor

Monitor the growth of the culture by optical density measurement.

identification

Identify your cultures by mass spectrometry or 16s rRNA sequencing.

Description

After cultivation there is enough material to study the cell´s phenotype.

Appnote

Here you can find our Appnote