A critical step in the process of isolating and studying microbes from complex communities is efficient separation of single community members for obtaining pure cultures. This is complicated by the uneven distribution and high complexity of mixed microbial samples such those from human feces, or from environmental sources like marine samples or waste water.
Traditionally this is done by colony streaking on agar plates or dilution-to-extinction. Using these methods, it is often difficult to obtain pure cultures due to the unknown composition and heterogeneous cell replication rates. Therefore we developed the b.sight, which enables automated and label-free single-cell isolation from complex microbial samples.
typical work flow
Prepare a single-cell suspension from your microbiome sample
Isolate your cells without bias directly into liquid growth media.
Incubate the isolated single-cells to obtain pure clonal cultures.
Monitor the growth of the culture by optical density measurement.
Identify your cultures by mass spectrometry or 16s rRNA sequencing.
After cultivation there is enough material to study the cell´s phenotype.
Here you can find our Appnote