Request records of the April 2020 cytena live webinar series

In addition to driving advancement in cell line development and single-cell genomics, cytena aims to keep the scientific community informed about new technologies and more efficient ways of working. Join us for a newly launched series of webinars on how single-cell dispensing improves workflow efficiency so you can get a greater number of single cells in less time with assured clonality and a higher rate of recovery.

Presented by Dr. Julian Riba, CSO
Live webinar took place April 9th, 2020
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Learn about:

  • Single-cell dispensing technology
  • Setting up highly efficient CLD workflows
  • Using the c.bird to bring suspension culture to 96-well plates

 

 

For more information read here:

Demonstrating monoclonality and maintaining cell viability in cell line development are prerequisites for efficient development of cell lines for producing therapeutic proteins. The first part of this session focusses on cytena´s single-cell dispensing technology and on setting up an automated and efficient CLD workflow for generation of high-quality clones.
In the second part of the session we will introduce the c.bird, a new 96-well microbioreactor for early suspension culture. We will discuss how the c.bird results in higher cell densities and increased protein yield early in the cell line development process, and ultimately, saves time and cost.
In 2020, the majority of top pharma companies used cytena’s single-cell dispensing technology to ensure fast antibody development.

 

 

 

Presented by Dr. Julian Riba, CSO
Live webinar took place April 16th, 2020
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Learn about:

  • Automated isolation of bacteria and other microorganisms
  • High-throughput cultivation workflow for isolating anaerobic bacteria from gut microbiota
  • Other research applications for the b.sight

 

For more information read here:

Gut microbiota are very diverse communities of microorganisms that reside in the gastrointestinal tract and play an important role in regulating the health of the host. Cultivation-based studies are essential because they facilitate proper taxonomic description of novel microbes and open avenues for downstream functional studies. In this session we discuss a simple workflow for rapid generation of pure bacterial cultures isolated from complex gut samples. We will demonstrate how the b.sight, a new instrument for single-cell dispensing of bacteria, can be used to rapidly sort single microbial cells and enable direct generation of clonal cultures. cytena´s b.sight technology allows researchers to bypass low efficiency “dilution-to-extinction” or agar streaking steps and can be easily integrated into anaerobic and automated workflows.

  

Presented by Dr. Julian Riba, CSO
Live webinar took place April 23rd, 2020
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Learn about:

  • Depositing individual mammalian and microbial cells  
  • Setting up protocols for low volume reagent dispensing and NGS library barcoding
  • Automation and miniaturization of smart-seq, a full-length single-cell transcript sequencing workflow

 

For more information read here:

Despite significant technological advances in microfluidic technologies, plate-based single-cell omics workflows offer the highest flexibility and unmatched assay sensitivity. This session introduces a new high-throughput workflow for generating sequencing libraries from single cells sorted into 384-well plates. First, we will discuss how cytena´s x.sight instruments can be used to precisely deposit single cells into well plates prefilled with sub-microliter amounts of lysis buffer. Second, we introduce the I-DOT, a new non-contact liquid handler that allows for rapid and highly multiplexed low volume reagent dispensing. Using this platform, the popular smart-seq protocol was performed at 10-fold reduced reaction volumes, including cDNA amplification, tagmentation, and library indexing.