cell line development
Clonal cell line development is a crucial step in various applications including generating biopharmaceuticals (e.g. monoclonal antibodies). Current workflows in cell line development have major drawbacks such as missing proof of clonality, inefficient single-cell isolation and reduced cell viability.
The single-cell printer™ technology offers documented assurance of clonality and provides efficient and fast single-cell seeding combined with excellent cell viability and zero risk of cross-contamination.
All cytena products support SLAS/SBS format 96-well and 384-well plates. A great variety of typical cell lines used in cell line development such as CHO-K1, HEK 293, L929 can be processed.
Discover our cell dispensing technology for your clonal cell line application.
typical CLD work flow
transfection
Include the producing gene into the host cell, generating viable, stable cells.
isolation
Isolate sufficiently many single cells in short time, with high purity and high viability.
growth
Recovery, which means deriving clonal colonies from the isolated single cells
stability
Achieve a stable cell colony over time with regard, to growth and product.
identification
Find the "best producer". Best in growth, stability and amount of product.
expansion
Further expand the colonies of interest in bioreactors. Keep them stable.
product
Harvest sufficient amount of product with highest purity. Cells are disposed.
proof
Document the entire process and such proof important facts about the product.
