cell line development

cell line development

Clonal cell line development is a crucial step in various applications including generating biopharmaceuticals (e.g. monoclonal antibodies). Current workflows in cell line development have major drawbacks such as missing proof of clonality, inefficient single-cell isolation and reduced cell viability.

The single-cell printer™ technology offers documented assurance of clonality and provides efficient and fast single-cell seeding combined with excellent cell viability and zero risk of cross-contamination.

All cytena products support SLAS/SBS format 96-well and 384-well plates. A great variety of typical cell lines used in cell line development such as CHO-K1, HEK 293, L929 can be processed.

Discover our cell dispensing technology for your clonal cell line application.

typical CLD work flow

transfection

Include the producing gene into the host cell, generating viable, stable cells.

isolation

Isolate sufficiently many single cells in short time, with high purity and high viability.

growth

Recovery, which means deriving clonal colonies from the isolated single cells

stability

Achieve a stable cell colony over time with regard, to growth and product.

identification

Find the "best producer". Best in growth, stability and amount of product.

expansion

Further expand the colonies of interest in bioreactors. Keep them stable.

product

Harvest sufficient amount of product with highest purity. Cells are disposed.

proof

Document the entire process and such proof important facts about the product.

assurance of clonality

Images taken during the selection and isolation process of single-cells.

before cell dispensing, tracking a …

… cells' way towards nozzle

targeted single-cell

targeted single-cell with contour overlay

after cell dispensing, confirmation that cell has left the nozzle